The insertion of genetic material into cells for therapeutic purposes is known as gene therapy. Stem cells are cells that can self-renew indefinitely and have the ability to differentiate into intermediate and mature cells. Adult stem cells, also known as tissue stem cells, create cells that are unique to the tissue in which they are located. They are relatively unspecialized, and when they differentiate, they are predestined to give rise to specific cell types. Different stem cell populations may be limited to specific developmental stages or cell types. The initial goal of gene therapy was to treat inherited genetic disorders by replacing a mutated gene with a functional one. On the other hand, stem cells were largely used for tissue repair. However, these technologies have a lot of overlap in their applications. In the treatment of hereditary and acquired disorders, both gene and stem cell therapies can be used. When two techniques are combined, their therapy potential is maximized. Gene therapy and stem cell research have grown in prominence in recent years.