International Conference On Orphan Drugs and Rare Diseases

August 17-19, 2023

August 17 -19, 2023 | Online Event
Orphan Drugs 2023

Arti Sharma

Speaker at Orphan Drugs and Rare Diseases 2023 - Arti Sharma
Govt. P.G. College Prithvipur, India
Title : Immunoproteomic approach towards vaccine development against Botulism of C botulinum Type B


Botulism is a neuroparalytic disease caused by botulinum neurotoxins (BoNTs) and has been reported as a potential bio warfare agent due to its extreme toxicity (~100 billion times more toxic than cyanide). BoNTs is produced by Gram positive; an obligate anaerobic and endospore forming bacteria called Clostridium botulinum. There is no licensed vaccine available commercially for the treatment of botulism. An immunoproteomic-based approach was used to screen the immunogenic proteins of C. botulinum type B. The identity of immunogenic proteins is important to develop the vaccine candidates and diagnostic markers against the botulism. Using a proteomics-based approach, whole cell proteome were separated by two-dimensional gel electrophoresis and immunoreactive proteins were revealed by reaction with antisera against whole cell proteins of C. botulinum type B. The identity of immunoreactive proteins were recognized by mass spectrometry. Fifty six pre dominant proteins were identified in total. Out of total proteins, 13- proteins were identified as immunogenic. These proteins may be potential vaccine / diagnostic candidate molecule against botulism.


I am working as assistant professor in Govt. P.G. College Prithvipur, India. I have published national and international papers in immunoproteomics. I had also worked in Defence Research & Development Establishment, Gwalior. I had developed diagnostic system as well as vaccine markers against botulism. I had also worked on Biodiesel in Indian institute of Technology, Roorkee, India as research scientist.